Application Note

Detection of pressure-induced protein aggregation using Microfluidic Modulation Spectroscopy (MMS)

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Application Note

The ability to detect protein aggregation is important at all stages of drug development. Early detection of aggregation is most desirable to inform development decisions since aggregation can negatively affect the functionality of a protein.

This Application Note Includes:

  • Highlights the significant changes present in the spectral regions of 1624 and 1640cm-1 in the Second Derivative plot that can be seen only subtly in the Absolute Absorbance spectral plot.
  • Weighted Spectral Difference (WSD) as a metric used to monitor change in sample secondary structure for this aggregation study
  • Strong linear correlation between increasing amounts of percent aggregation and increasing amounts of measured antiparallel beta-sheet content

Pressure-Induced Protein Aggregation Detection Protein aggregation is a recognized signal of instability and can lead to the loss of protein function. It is therefore crucial to detect protein aggregation early in the drug development process to inform further drug development decisions. Pressure, a stressor used for generating aggregates by impacting noncovalent interactions without the need to change temperature or solvents1, was employed to create aggregated protein for this spiked study.

Change How You See Biomolecules

Microfluidic Modulation Spectroscopy provides a powerful alternative, producing consistently reproducible data that goes beyond that supplied by current techniques. The high-performance fully automated AQS3pro system and AQS3delta analytics package allow formulation and discovery scientists to quickly and easily run samples, process data and gain insight.


Next generation in protein structure determination

The AQS³pro powered by Microfluidic Modulation Spectroscopy is the next generation infrared based spectroscopy platform for protein structure determination. It represents a novel tool that will strengthen you bioanalytical tool kit and provide critical insights into protein based drug development.

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