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Application Note

Thermal Denaturation Analysis of Bovine Serum Albumin over Wide Concentration Range by Microfluidic Modulation Spectroscopy

This Application Note Includes:

In this App, MMS was used to study the heat-induced thermal denaturation of BSA samples
Allows the use of simpler detectors without the need for liquid nitrogen cooling
Differentiates small changes in secondary structure across a wide concentration range without the interference of excipients or loss of linearity at the lowest and highest concentrations
Delivers information about aggregation, stability, HOS, and similarity in addition to quantitation results

Secondary Structure Analysis Tools For Measuring Thermal Denaturation

Microfluidic Modulation Spectroscopy (MMS) is a revolutionary new technology capable of highly sensitive and reproducible measurements of protein secondary structure using IR absorbance in the Amide I band (1700-1600 cm-1). MMS is performed on the AQS3pro system from RedShift BioAnalytics, and it generates measurements over a wide range of complex backgrounds, including buffers that contain absorbing materials and scattering components such as reducing agents, adjuvants, surfactants, and other excipients.

Change How You See Biomolecules

Microfluidic Modulation Spectroscopy provides a powerful alternative, producing consistently reproducible data that goes beyond that supplied by current techniques. The high-performance fully automated AQS3pro system and AQS3delta analytics package allow scientists to quickly and easily run samples, process data and gain insight.

AQS3pro

Next generation in protein structure determination.

The AQS³pro powered by Microfluidic Modulation Spectroscopy is the next generation infrared based spectroscopy platform for protein structure determination. It represents a novel tool that will strengthen you bioanalytical tool kit and provide critical insights into protein based drug development.

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