Application Note

Monoclonal Antibody Analysis by Microfluidic Modulation Spectroscopy in a Complex Formulation Buffer 

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Application Note

This Application Note Includes:

  • A native monoclonal antibody was measured by MMS in a complex buffer formulation at concentrations ranging from 1 to 150 mg/mL
  • The use of simpler detectors without the need for liquid nitrogen cooling
  • Demonstration of how MMS automatically introduces the sample protein solution and a matching reference buffer stream into a microfluidic flow cell
  • How MMS modulates two fluids rapidly across the  Quantum Cascade Laser beam path,  producing nearly drift-free background compensated measurements 

Monoclonal Antibodies Secondary Structure Determination

Microfluidic Modulation Spectroscopy (MMS) is a powerful infrared spectroscopy tool for protein structural analysis developed by RedShift BioAnalytics. This technology provides significant increases in sensitivity, dynamic range, and accuracy for determination of protein secondary structure relative to conventional mid-IR and far-UV CD techniques. This application note demonstrates the utility of the technique for the determination of monoclonal antibodies secondary structure at high buffer concentrations.

Change How You See Biomolecules

Microfluidic Modulation Spectroscopy provides a powerful alternative, producing consistently reproducible data that goes beyond that supplied by current techniques. The high-performance fully automated AQS3pro system and AQS3delta analytics package allow scientists to quickly and easily run samples, process data and gain insight.


Next generation in protein structure determination.

The AQS³pro powered by Microfluidic Modulation Spectroscopy is the next generation infrared based spectroscopy platform for protein structure determination. It represents a novel tool that will strengthen you bioanalytical tool kit and provide critical insights into protein based drug development.
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Learn how industry leaders are using our MMS technology in their lab.


Read the latest peer-reviewed publications on our MMS technology.

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