Application Note

Microfluidic Modulation Spectroscopy (MMS) Fills an Analytical Gap with a Lower LOQ for Measuring Protein Misfolding and Structural Similarity 

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Application Note

This Application Note Includes:

  • MMS was used to study the heat-induced thermal denaturation of BSA samples
  • Allows the use of simpler detectors without the need for liquid nitrogen cooling
  • Differentiates small changes in secondary structure across a wide concentration range without the interference of excipients or loss of linearity at the lowest and highest concentrations
  • Delivers information about aggregation, stability, HOS, and similarity in addition to quantitation results

Using MMS For Measuring Protein Misfolding And Aggregation

Protein misfolding in secondary structure can occur during all phases of drug development. A protein misfold represents a structural impurity and at any level can result in changed efficacy and increase the potential for immunogenicity. Improving spectroscopic methods for measuring low level impurities in secondary structure is necessary to maintain confidence in a protein’s integrity during all phases of drug development. Common structural characterization methods such as FTIR and CD have known limitations in reproducibility and sensitivity which adversely increase the lowest level of quantitation (LOQ) achievable when measuring structural impurities and similarity. Microfluidic Modulation Spectroscopy is a protein characterization method which generates reproducible high resolution measurements. 

Change How You See Biomolecules

Microfluidic Modulation Spectroscopy provides a powerful alternative, producing consistently reproducible data that goes beyond that supplied by current techniques. The high-performance fully automated AQS3pro system and AQS3delta analytics package allow scientists to quickly and easily run samples, process data and gain insight.


Next generation in protein structure determination.

The AQS³pro powered by Microfluidic Modulation Spectroscopy is the next generation infrared based spectroscopy platform for protein structure determination. It represents a novel tool that will strengthen you bioanalytical tool kit and provide critical insights into protein based drug development.
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