This Application Note Includes:
Protein misfolding in secondary structure can occur during all phases of drug development. A protein misfold represents a structural impurity and at any level can result in changed efficacy and increase the potential for immunogenicity. Improving spectroscopic methods for measuring low level impurities in secondary structure is necessary to maintain confidence in a protein’s integrity during all phases of drug development. Common structural characterization methods such as FTIR and CD have known limitations in reproducibility and sensitivity which adversely increase the lowest level of quantitation (LOQ) achievable when measuring structural impurities and similarity. Microfluidic Modulation Spectroscopy is a protein characterization method which generates reproducible high resolution measurements.
Next generation in protein structure determination.